Flow Cytometry: First Principles, Second Edition By Alice Longobardi Givan(auth.)
2001 | 280 Pages | ISBN: 0471382248 | PDF | 5 MB
Flow cytometry continually amazes scientists with its ever-expanding utility. Advances in flow cytometry have opened new directions in theoretical science, clinical diagnosis, and medical practice. The new edition of Flow Cytometry: First Principles provides a thorough update of this now classic text, reflecting innovations in the field while outlining the fundamental elements of instrumentation, sample preparation, and data analysis. Flow Cytometry: First Principles, Second Edition explains the basic principles of flow cytometry, surveying its primary scientific and clinical applications and highlighting state-of-the-art techniques at the frontiers of research. This edition contains extensive revisions of all chapters, including new discussions on fluorochrome and laser options for multicolor analysis, an additionalsection on apoptosis in the chapter on DNA, and new chapters onintracellular protein staining and cell sorting, including high-speed sorting and alternative sorting methods, as well as traditional technology. This essential resource: Assumes no prior knowledge of flow cytometry Progresses with an informal, engaging lecture style from simpleto more complex concepts Offers a clear introduction to new vocabulary, principles of instrumentation, and strategies for data analysis Emphasizes the theory relevant to all flow cytometry, with examples from a variety of clinical and scientific fields Flow Cytometry: First Principles, Second Edition provides scientists, clinicians, technologists, and students with the knowledge necessary for beginning the practice of flow cytometry and for understanding related literature.Content: Chapter 1 The Past as Prologue (pages 1–9): Chapter 2 Setting the Scene (pages 11–14): Chapter 3 Instrumentation: Into the Black Box (pages 15–39): Chapter 4 Information: Harnessing the Data (pages 41–57): Chapter 5 Seeing the Light: Lasers, Fluorochromes, and Filters (pages 59–80): Chapter 6 Cells from Without: Leukocytes, Surface Proteins, and the Strategy of Gating (pages 81–113): Chapter 7 Cells from Within: Intracellular Proteins (pages 115–122): Chapter 8 Cells from Within: DNA in Life and Death (pages 123–158): Chapter 9 The Sorting of Cells (pages 159–174): Chapter 10 Disease and Diagnosis: The Clinical Laboratory (pages 175–194): Chapter 11 Out of the Mainstream: Research Frontiers (pages 195–223): Chapter 12 Flowing On: The Future (pages 225–228):
Download File Size:3.89 MB
|
