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"Cell Culture" ed. by Radwa Ali Mehanna
ITExLi | 2019 | ISBN: 1789848679 9781789848670 1789848660 9781789848663 | 275 pages | PDF | 22 MB
This book covers some advanced aspects in cell culture methodologies. The book provides a comprehensive overview of some of the advanced cell culture methodologies and applications. It serves as a valuable source for scientists, researchers, clinicians and students.
Cell culture is one of the major tools used in cellular and molecular biology, delivering an excellent model for studying the normal physiology and biochemistry of cells.
The book has four sections discussing different types of cell culture models, including 3D cell culture techniques, their advantages, and limitations in comparison to traditional 2D culturing; cell viability, autophagy, in vitro toxicity tests and live cell imaging; stem cell culture for cell-based therapeutics; and specific applications and methodologies for hybrid cell lines and cancer models.
Contents
1 2D vs. 3D Cell Culture Models for In Vitro Topical (Dermatological) Medication Testing
2 Two-Dimensional (2D) and Three-Dimensional (3D) Cell Culturing in Drug Discovery
3 Time-Lapse Microscopy
4 Cell-Based Assays for Evaluation of Autophagy in Cancers
5 In Vitro Toxicity Testing of Nanomaterials
6 Culturing Adult Stem Cells for Cell-Based Therapeutics: Neuroimmune Applications
7 Morphological Comparison of Stem Cells Using Two-Dimensional Culture and Spheroid Culture
8 Authenticating Hybrid Cell Lines
9 Air Pouch Model: An Alternative Method for Cancer Drug Discovery
10 Optimization of the Self-Assembly Method for the Production of Psoriatic Skin Substitutes
11 Monolayers of Carbohydrate-Containing Lipids at the Water-Air Interface
12 Erythropoiesis and Megakaryopoiesis in a Dish
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